James Fellows Yates (james_fellows_yates@eva.mpg.de)
2020-09-04 13:21:22

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2020-09-04 13:21:27

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James Fellows Yates (james_fellows_yates@eva.mpg.de)
2020-09-08 08:34:15

Becky brought up this paper: https://aem.asm.org/content/80/9/2648

It reports a viral metagenome (sequenced on 454...) from a C14th coprolite. While it is reported as a 'metagenome' it looks like it was 'selected' so to say for small particles via filtering but then it was shotgun sequenced... aside from me being highly skeptical of the quality do you think it warrants conclusion...?

James Fellows Yates (james_fellows_yates@eva.mpg.de)
2020-09-08 08:34:36

Arguably our extraction methods also include 'size filtering' as the protocols are optmised to recover short reads...

ivelsko (velsko@shh.mpg.de)
2020-09-08 14:34:08

I think it can count b/c that’s a typical way to filter for viruses in a sample, and they have a bunch of different kinds of viruses

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2020-09-10 09:56:03

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2020-09-22 19:52:53

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James Fellows Yates (james_fellows_yates@eva.mpg.de)
2021-01-19 20:17:22

@ivelsko https://github.com/SPAAM-community/AncientMetagenomeDir/pull/105 can we resolve this now as well with the info from Ana Phillips?

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ivelsko (velsko@shh.mpg.de)
2021-01-20 09:18:34

Yep we can

👍 James Fellows Yates
James Fellows Yates (james_fellows_yates@eva.mpg.de)
2021-01-20 09:19:46

When you have a moment can you do a cross-check? See if there are any duplicate samples with your original PR and that the IDs are standardised with your PR as well?

James Fellows Yates (james_fellows_yates@eva.mpg.de)
2021-01-20 09:20:15

If there are any differences I can to through and fix stuff against the sheet you have

ivelsko (velsko@shh.mpg.de)
2021-01-20 11:15:58

There’s only 1 sample for Philips2020, and I checked it against the 2017 entries. I updated the lat/long, but that’s the only change. Since this is also old, do you want me to make a new PR instead?

James Fellows Yates (james_fellows_yates@eva.mpg.de)
2021-01-20 11:16:10

*Thread Reply:* Yes please!

ivelsko (velsko@shh.mpg.de)
2021-01-20 11:36:22

*Thread Reply:* Done!

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